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Journal: bioRxiv
Article Title: Tracking long-term functional connectivity maps in human stem-cell-derived neuronal networks by holographic-optogenetic stimulation
doi: 10.1101/2021.05.11.443589
Figure Lengend Snippet: (A) Experimental scheme of iNGN-astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron-glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin-coated MEA chambers at 15 dpi. ChR2-EYFP-labeled cells are visualized by live cell microscopy. Scale bars, 100 µm. mTeSR1: standardized medium for the feeder-independent maintenance of hiPSCs. BrainPhys: serum-free neurophysiological basal medium for improved neuronal function. LV-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.
Article Snippet: Then,
Techniques: Co-Culture Assay, Functional Assay, Microscopy, Imaging, Labeling