complete brainphys neuronal medium Search Results


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STEMCELL Technologies Inc brainphys™ neuronal medium
(A) Experimental scheme of iNGN-astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron-glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin-coated MEA chambers at 15 dpi. ChR2-EYFP-labeled cells are visualized by live cell microscopy. Scale bars, 100 µm. mTeSR1: standardized medium for the feeder-independent maintenance of hiPSCs. <t>BrainPhys:</t> serum-free neurophysiological basal medium for improved neuronal function. LV-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.
Brainphys™ Neuronal Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brainphys™ neuronal medium/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
brainphys™ neuronal medium - by Bioz Stars, 2026-03
90/100 stars
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90
iCell Gene Therapeutics brainphys neuronal medium
(A) Experimental scheme of iNGN-astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron-glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin-coated MEA chambers at 15 dpi. ChR2-EYFP-labeled cells are visualized by live cell microscopy. Scale bars, 100 µm. mTeSR1: standardized medium for the feeder-independent maintenance of hiPSCs. <t>BrainPhys:</t> serum-free neurophysiological basal medium for improved neuronal function. LV-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.
Brainphys Neuronal Medium, supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/brainphys neuronal medium/product/iCell Gene Therapeutics
Average 90 stars, based on 1 article reviews
brainphys neuronal medium - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc serum-free neuronal basal brainphys™ neuronal medium
(A) Experimental scheme of iNGN-astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron-glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin-coated MEA chambers at 15 dpi. ChR2-EYFP-labeled cells are visualized by live cell microscopy. Scale bars, 100 µm. mTeSR1: standardized medium for the feeder-independent maintenance of hiPSCs. <t>BrainPhys:</t> serum-free neurophysiological basal medium for improved neuronal function. LV-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.
Serum Free Neuronal Basal Brainphys™ Neuronal Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serum-free neuronal basal brainphys™ neuronal medium/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
serum-free neuronal basal brainphys™ neuronal medium - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


(A) Experimental scheme of iNGN-astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron-glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin-coated MEA chambers at 15 dpi. ChR2-EYFP-labeled cells are visualized by live cell microscopy. Scale bars, 100 µm. mTeSR1: standardized medium for the feeder-independent maintenance of hiPSCs. BrainPhys: serum-free neurophysiological basal medium for improved neuronal function. LV-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.

Journal: bioRxiv

Article Title: Tracking long-term functional connectivity maps in human stem-cell-derived neuronal networks by holographic-optogenetic stimulation

doi: 10.1101/2021.05.11.443589

Figure Lengend Snippet: (A) Experimental scheme of iNGN-astrocyte co-culture preparation (left) and experimental procedure (right) over time. Functional recordings, microscopy imaging, full-field and holographic stimulations were studied on different days post induction (dpi). (B) Schematic of the so-called Banker culture, a neuron-glia co-culture system within the MEA device. (C) Representative microscopy images of iNGN cells. Upper panel, iNGN cells grown on Matrigel-coated well plates at 3 dpi. Lower panel, PDL-laminin-coated MEA chambers at 15 dpi. ChR2-EYFP-labeled cells are visualized by live cell microscopy. Scale bars, 100 µm. mTeSR1: standardized medium for the feeder-independent maintenance of hiPSCs. BrainPhys: serum-free neurophysiological basal medium for improved neuronal function. LV-ChR2-EYFP: lentiviral particles delivering ChR2-EYFP to iNGN cells. Dox: doxycycline. Ara-C: cytosine arabinoside to remove undifferentiated cells.

Article Snippet: Then, complete BrainPhys™ (BrainPhys™ Neuronal Medium (Stemcell Technologies) + 1% P/S + NeuroCult™ SM1 Neuronal Supplement (Stemcell Technologies) + N2 Supplement-A + 20 ng/ml recombinant human BDNF (Peprotech) + 20 ng/ml recombinant human GDNF (Peprotech) + 200 nM ascorbic acid (Sigma)) was added to the MEA rings, covering neuronal and astrocyte cultures ( ).

Techniques: Co-Culture Assay, Functional Assay, Microscopy, Imaging, Labeling